CURRICULUM VITAE

Tosifusa Toda, Ph.D.

Professor of Yokohama City University

Mailing Address:

　　　　　　　 Advanced Medical Research Center

　　　　　　　 Yokohama City University

　　　　　　　 Fukuura 3-9, Kanazawa,

　　　　　　　 Yokohama 236-0004 , Japan

　　　　　　　 Tel:　 81-45-787-2791

　　　　　　　 E-mail: ttoda@yokohama-cu.ac.jp

Education:

　　　　　　　 Ph.D. Osaka University, 1988 (Major field: Biological Chemistry)

　　　　　　　 Osaka University, Graduate School of Science, 1967-1970

　　　　　　　 Osaka University, School of Science, 1971-1975

　　　　　　　 Fujishima Senior High School, 1967-1970

Awards:

　　　　　　　 The 22nd Kodama Memorial Award from the Japanese

　　　　　　　 Electrophoresis Society, 1983

　　　　　　　 The 2nd International Exchange Promoting Award from the

　　　　　　　 Japanese Electrophoresis Society, 2005

Work Experiences:

　　　　　　　 1977-1988　 Tokyo Metropolitan Institute of Gerontology,

　　　　　　　　　　　　　　　 Laboratory of Biochemical Research, Assistant Researcher

　　　　　　　 1988-1989　 University of California, San Francisco, Laboratory of

　　　　　　　　　　　　　　　 Dermatology Research, Visiting Research Scientist

　　　　　　　 1988-2002　 Tokyo Metropolitan Institute of Gerontology,

　　　　　　　　　　　　　　　 Laboratory of Molecular Biology, Senior Research Scientist

　　　　　　　 2002-2005　 Tokyo Metropolitan Institute of Gerontology,

　　　　　　　　　　　　　　　 Collaborative Research Group for Proteomics

　　　　　　　 2005-2011　 Tokyo Metropolitan Institute of Gerontology, Research

　　　　　　　　　　　　　　　 Team for Molecular Biomarkers, Proteomic Gerontology

　　　　　　　　　　　　　　　 Research Unit, Leader

　　　　　　　 2011- present 　Yokohama City University, Professor

Affiliations to Academic Societies:

　　　　　　　 Japanese Electrophoresis Society, Vice-president

　　　　　　　 Japan Human Proteome Organization, Council Member

　　　　　　　 Japan Society for Biomedical Gerontology, Council Member

　　　　　　　 Japanese Biochemical Society, Member

　　　　　　　

　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　

Latest Major Publications:

"Proteomic analysis of Epstein-Barr virus-transformed human B-lymphoblastoid cell lines before and after immortalization"

Toda, T., Sugimoto, M., Omori, A., Matsuzaki, T., Furuichi, Y., Kimura, N. (2000) Electrophoresis, 21 (9), 1814-1822.

"An extra human chromosome 21 reduces mlc-2a expression in chimeric mice and Down syndrome"

Nishigaki R, Shinohara T, Toda T, Omori A, Ichinose S, Itoh M, Shirayoshi Y, Kurimasa A, Oshimura M. (2002) Biochem Biophys Res Commun. 295(1), 112-118.

"Proteomic approach to determination of the significance of protein oxidation in the ageing of mouse hippocampus"

Toda, T., Morimasa, T., Kobayashi, S., Nomura, K., Hatozaki T., and Hirota, M. (2003) Appl Genomics Proteomics 2(1), 43-50

"Proteomics-based identification of differentially expressed genes in human gliomas: down-regulation of SIRT2 gene"

Hiratsuka M, Inoue T, Toda T, Kimura N, Shirayoshi Y, Kamitani H, Watanabe T, Ohama E, Tahimic CG, Kurimasa A, Oshimura M. (2003) Biochem Biophys Res Commun. 309(3), 558-566.

"Citrullination preferentially proceeds in glomerular Bowman's capsule and increases in obstructive nephropathy."

Feng D, Imasawa T, Nagano T, Kikkawa M, Takayanagi K, Ohsawa T, Akiyama K, Ishigami A, Toda T, Mitarai T, Machida T, Maruyama N. (2005) Kidney Int, 68, 84-95.

"Proteomic identification of differentially-expressed genes in human gastric carcinomas."

Nishigaki R, Osaki M, Hiratsuka M, Toda T, Murakami K, Jeang KT, Ito H, Inoue T, Oshimura M. (2005) Proteomics, 5, 3205-3213.

"Collaborative proteomics framework with XML databases and an integrated XML viewer for 2DPAGE."

Morisawa H, Toda T. (2005) J Electrophoresis, 49(1), 35-38.

"Age-dependent variations of cell response to oxidative stress: proteomic approach to protein expression and phosphorylation."

Miura Y, Kano M, Abe K, Urano S, Suzuki S, Toda T. (2005) Electrophoresis, 26(14), 2786-2796.

"Comparative proteomics of flotillin-rich Triton X-100-insoluble lipid raft fractions of mitochondria and synaptosome from mouse brain."

Nakamura M, Sakurai Y, Takeda Y, Toda T., (2005) J Electrophoresis, 49(4), 77-83.

"Proteomic Study on the variation of protein expression and phosphorylation caused by radiation in culture grial cells."

Miura Y. Kano M, Toda T, Suzuki S. (2005) Biol Sci Space, 19(2), 98-99.

"Abnormal accumulation of citrullinated proteins catalyzed by peptidylarginine deiminase in hippocampal extracts from patients with Alzheimer's disease."

Ishigami A, Ohsawa T, Hiratsuka M, Taguchi H, Kobayashi S, Saito Y, Murayama S, Asaga H, Toda T, Kimura N, Maruyama, N. (2005) J. Neurosci. Res., 80, 120-128.

"Proteomic analysis on insulin signaling in human hematopoietic cells: identification of CLIC1 and SRp20 as novel downstream effectors of insulin."

Saeki K, Yasugi E, Okuma E, Breit SN, Nakamura M, Toda T, Kaburagi Y, Yuo A. (2005) Am. J Physiol. Endocrinol. Metab., 289(3), E419-428.

"Phosphoproteomic profiling of human SH-SY5Y neuroblastoma cells during response to 6-hydroxydopamine-induced oxidative stress."

Nakamura M, Yamada M, Ohsawa T, Morisawa H, Nishine T, Nishimura O, Toda T. (2006) Biochem. Biophys. Acta, 1763(9), 977-989.

"Analysis of protein-protein interactions with a multi-capillary electrophoresis instrument."

Shimura K, Waki T, Okada M, Toda T, Kimoto I, Kasai K. (2006) Electrophoresis, 27, 1886-1894.

"Increased expression and phosphorylation of liver glutamine synthetase in well-differentiated hepatocellular carcinoma tissues from patients infected with hepatitis C virus."

Kuramitsu Y, Harada T, Takashima M, Yokoyama Y, Hidaka I, Iizuka N, Toda T, Fujimoto M, Zhang X, Sakaida I, Okita K, Oka M, Nakamura K. (2006) Electrophoresis, 27(8), 1651-1658.

"Identification and characterization of an increased glycoprotein in aging: age-associated translocation of cathepsin D."

Sato Y, Suzuki Y, Ito E, Shimazaki S, Ishida M, Yamamoto T, Yamamoto H, Toda T, Suzuki M, Suzuki A, Endo T. (2006) Mech. Ageing Dev., 127(10), 771-778.

"The protein expression profile of cynomolgus monkey embryonic stem cells in two-dimensional gel electrophoresis: a successful identification of multiple proteins using human databases."

Nakahara M, Saeki K, Yogiashi Y, Kimura A, Horiuchi A, Nakamura N, Yoneda A, Saeki K, Matsuyama S, Nakamura M, Toda T, Kondo Y, Kaburagi Y, Yuo A. (2007) J. Electrophoresis, 51(1), 1-8.

"Proteomic Study on X-irradiation-responsive Proteins and Ageing: Search for Responsible Proteins for Radiation Adaptive Response."

Miura Y, Kano M, Yamada M, Nishine T, Urano S, Suzuki S, Endo T, Toda T. (2007) J. Biochem. (Tokyo)., 142(2), 145-155.

"Proteomic identification of oxidative-stress-reporting biomarkers differentially secreted from human neuroblastoma SH-SY5Y cells."

Toda T, Nakamura M, Morisawa H, Hirota M. (2007) J. Electrophoresis, 51(3/4), 21-26.

"A comparison study in the proteomic signatures of multipotent germline stem cells, embryonic stem cells, and germline stem cells."

Kurosaki H, Kazuki Y, Hiratsuka M, Inoue T, Matsui Y, Wang, CC, Kanatsu-Shinohara M, Shinohara T, Toda T., Oshimura M. (2007) Biochem. Biophys. Res. Comm., 353, 259-267.

"Proteomic identification of differentially expressed genes in mouse neural stem cells and neurons differentiated from embryonic stem cells in vitro."

Akama K, Tatsuno R, Otsu M, Horikoshi T, Nakamura M, Toda T, Inoue N. (2008) Biochim. Biophys. Acta, 1784, 773-782.

"Analysis of Glycopeptides Using Lectin Affinity Chromatography with MALDI-TOF Mass Spectrometry"

Kubota K, Sato Y, Suzuki Y, Goto-Inoue N, Toda T, Suzuki M, Hisanaga S, Suzuki A, Endo T. (2008) Anal. Chem., 80 (10), 3693–3698.

"Glycoproteomic analysis of abnormal N-glycosylation on the kappa chain of cryocrystalglobulin in a patient of multiple myeloma."

Toda T, Nakamura M, Yamada M, Nishine T, Torii T, Ikenaka K, Hashimoto R, Mori M. (2009) J. Electrophoresis, 53(1), 1-6.

"Proteomic analysis of protein complexes in human SH-SY5Y neuroblastoma cells by using blue-native gel electrophoresis: An increase in lamin A/C associated with heat shock protein 90 in response to 6-hydroxydopamine-induced oxidative stress."

Nakamura M, Morisawa H, Imajoh-Ohmi S, Takamura C, Fukuda H, Toda T. (2009) Exp. Gerontol. 44(6-7), 375-382.

"Involvement of Galectin-3 with Vascular Cell Adhesion Molecule-1 in Growth Regulation of Mouse BALB/3T3 cells."

Tadokoro T, Ikekita M, Toda T, Ito H, Sato T, Nakatani R, Hamaguchi Y, Furukawa K. (2009) J. Biol. Chem. 284(51), 35556-35563.

"Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme."

Akama K, Horikoshi T, Sugiyama A, Nakahata S, Akitsu A, Niwa N, Intoh A, Kakui Y, Sugaya M, Takei K, Imaizumi N, Sato T, Matsumoto R, Iwahashi H, Kashiwabara SI, Baba T, Nakamura M, Toda T. (2010) Biochim Biophys Acta. 1804(6), 1272-1284.

"Proteomic analysis of plasma proteins in Japanese semisuper centenarians"

Miura Y, Sato Y, Arai Y, Abe Y, Takayama M, Toda T, Hirose N, Endo T. (2011) Exp Gerontol. 46, 81-85.

"Quantitative evaluation of lectin-reactive glycoforms of alpha1-acid glycoprotein using lectin affinity capillary electrophoresis with fluorescence detection."

Shimura K, Tamura M, Toda T, Yazawa S, Kassai K. (2011) Electrophoresis, 32(16), 2188-2193.

"Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including alpha-actinin 4."

Akimoto Y, Miura Y, Toda T, Wolfert MA, Wells L, Boons G-J, Hart GW, Endo T, Kawakami H. (2011) Clin Proteomics. 8(15), 1-16.

"Proteomic identification of differentially expressed genes during differentiation of cynomolgus monkey (Macaca fascicularis) embryonic stem cells to astrocyte progenitor cells in vitro."

Kuniko Akama, Tomoe Horikoshi, Takashi Nakayama, Masahiro Otsu, Noriaki Imaizumi, Megumi Nakamura, Tosifusa Toda, Michiko Inuma, Hisashi Hirano, Yasushi Kondo, Yutaka Suzuki, Nobuo Inoue (2013) Biochim Biophys Acta. 1834, 601-610.