Title 
       Changes in the cell surface of human diploid fibroblasts
       during cellular aging. 
Author 
       Yamamoto K; Yamamoto M 
Address 
       Department of Cell Biology, Tokyo Metropolitan Institute
       of Gerontology, Japan. 
Source 
       Mutat Res, 1991 Mar-Nov, 256:2-6, 169-75 
Abstract 
       The electrophoretic mobility of 13 human diploid cell
       strains, TIG-1, TIG-2, TIG-3, TIG-7, WI-38, IMR-90,
       MRC-5, MRC-9, TIG-1H, TIG-1L, TIG-2M, TIG-2B, and
       TIG-3S, which were established from different tissues of
       human embryos, was studied at different passages. The net
       negative surface charge of the cells was characteristic for
       each cell strain and decreased significantly during the in
       vitro aging of the cells. The decrease in the net negative
       charge of the cells correlated well with the decrease in
       cell density throughout the life span of the cells. A strict
       linear correlation between the electrophoretic mobility
       and the number of cells harvested at each passage was
       obtained for all the human diploid cell strains. Moreover,
       almost the same linear regression coefficient of the cells
       was obtained among these cell strains. Therefore, the net
       negative surface charge of human diploid cell strains could
       serve as a cell surface marker for in vitro cellular aging.