Title 
       Interferon-beta, an autocrine cytokine, suppresses human
       fetal skin fibroblast migration into a denuded area in a
       cell monolayer but is not involved in the age-related
       decline of cell migration. 
Author 
       Kondo H; Yonezawa Y; Ito H 
Address 
       Department of Experimental Biology, Tokyo Metropolitan
       Institute of Gerontology, Japan. 
Source 
       Mech Ageing Dev, 1996 Jun 25, 87:3, 141-53 
Abstract 
       The migration of human skin fibroblasts into a denuded
       area in a cell monolayer declined during in vitro and in vivo
       aging. We carried out a study to determine whether this
       age-related decline in cell migration was mediated by the
       autocrine cytokine interferon-beta (IFN-beta), which has
       been reported to suppress the proliferation, chemotaxis
       and collagen synthesis of human fibroblasts. Actually,
       IFN-beta specifically suppressed the migration of TIG-3S
       human fetal skin fibroblasts into a denuded area in a cell
       monolayer, as shown by the dose response experiments of
       IFN-beta and neutralizing anti-IFN-beta antibody. IFN-beta
       also inhibited their collagen synthesis but the addition of
       type I collagen could not reverse IFN-beta-induced
       inhibition of cell migration. Double strand RNA, which has
       been generally known to induce IFN-beta in human skin
       fibroblasts, suppressed the migration of TIG-3S cells.
       Next, a study was done to determine whether IFN-beta and
       double strand RNA suppressed the migration of TIG-3S
       cells in late passages as well as early passages, or
       whether neutralizing anti-IFN-beta antibody stimulated
       the migration of TIG-3S cells in late and middle passages.
       IFN-beta and double strand RNA suppressed the migration
       of TIG-3S cells in middle (PD45) and late (PD55) passages
       as well as in early passages (PD23-28). Neutralizing
       anti-IFN-beta antibodies could not reverse the low
       migratory activity of middle and late passage cells to the
       high migratory activity of early passage cells. These
       results indicated that the autocrine cytokine IFN-beta did
       not seem to be involved in the age-dependent decline of
       fibroblast migration.