TMIG Proteome Database for Research on Human Cell Aging Written in XML
(Under construction)
TIG-3, normal human diploid fibroblast cell strain
The cell strain TIG-3, which was established in our institute, has the finite life span up to 70-80 population doubling levels (PDLs). The cell strain has been widely used by many groups of researchers for investigating the molecular mechanisms of cellular aging and immortalization. The preparation of the 2-D gel proteome database was intended for promoting researches on cellular aging.
(Toda T, Kaji K, Kimura N. Electrophoresis 19(2), 344-348, 1998) (Toda T, Satoh M, Sugimoto M, Goto M, Furuichi Y, Kimura N. Mech Ageing Dev 100(2):133-143, 1998) ( Toda T. Ann N Y Acad Sci 928:71-78, 2001)
The clickable 2-D gel image map of the TIG-3 which is a normal human diploid fibroblast cell strain established in our institute. The colored markers indicate the quantitative variation of each spot protein in the course of aging.
IPG strip used: Immobiline DryStrip (18 cm), pH 4-7; Code No. 17-1233-01; Lot No. 236318
This database is presented by Toda, T., Kaji, K., and Kimura, N.