TMIG Proteome Database of Human Cell Aging in HTML

An alternative version of proteome database in XML is under construction

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TIG-3, normal human diploid fibroblast cell strain

The cell strain TIG-3, which was established in our institute, has the finite life span up to 70-80 population doubling levels (PDLs). The cell strain has been widely used by many groups of researchers for investigating the molecular mechanisms of cellular aging and immortalization. The preparation of the 2-D gel proteome database was intended for promoting researches on cellular aging. (Toda T, Kaji K, Kimura N. Electrophoresis 19(2), 344-348, 1998) (Toda T, Satoh M, Sugimoto M, Goto M, Furuichi Y, Kimura N. Mech Ageing Dev 100(2):133-143, 1998) (Toda T. Experimental Gerontology 35: 803-810, 2000 (PDF)) ( Toda T. Ann N Y Acad Sci 928:71-78, 2001)

The clickable 2-D gel image map of the TIG-3 which is a normal human diploid fibroblast cell strain established in our institute. The colored markers indicate the quantitative variation of each spot protein in the course of aging.
IPG strip used: Immobiline DryStrip (18 cm), pH 4-7; Code No. 17-1233-01; Lot No. 236318

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• The standard image map of colored spots indicating age-related protein variation
  
  
• The raw image for spot matching by an image analyzer
  
  

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• The list of spot protein data
  
  
• The search of spot protein data by ssp number
  
  
• The morphplogical alteration of TIG-3 cells with aging.
  
  

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Remarkable spot proteins which showed significant fluctuations


Spots increased

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Spots decreased

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Spots increased then decreased

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Refenceces on TIG-3

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Scatterplot of spot protein quantities in duplicate

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Skin-specific spots on Master 2-D Gel Protein Map of human diploid fibroblasts

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